Development of a 96-well drug model for skeletal muscle endogenous repair

Amir Reza Meysami Fard (1), Alison McGuigan (1, 2), Penney Gilbert (1), Maral Moosavi (1)

(1) Institute of Biomaterials and Biomedical Engineering, University of Toronto

(2) Department of Chemical Engineering, University of Toronto

Muscle endogenous repair occurs naturally in healthy individuals by recruiting muscle stem cells to proliferate and differentiate into new fibers. For people with muscular dystrophies or older individuals, the regenerative system does not work as properly. In order to develop effective treatments for these people, an in vitro model has been created that can successfully recapitulate the in vivo environment. It achieves this by successfully incorporating muscle stem cells into muscle fibers. Upon inducing injury, the muscle stem cells regenerate new fibers. My project focuses on making this model high-throughput by incorporating device design and advanced data-processing techniques; then utilizing this system to generate new targets.