Contribution of Pannexin-1 to 4-AP Seizures in Mouse Neocortex

Aquilino, Mark 1, 2; Whyte-Fagundes, Paige 2, 3; Zoidl, Georg 2, 3; Bardakjian, Berj 1; Carlen, Peter 1, 2

1. Institute of Biomaterials and Biomedical Engineering; 2. Krembil Research Institute - University Health Network; 3. York University

 Pannexin-1 (Panx1) has been shown to have a vital role in modulating neuronal excitability and in acute neurodegenerative changes. Through quantitative analysis of electrophysiological properties of C57Bl/6 mice with Panx1 either pharmacologically blocked or knocked out, new evidence into the dependence of the 4AP seizure model on Panx1 function is presented. Whole-cell in vitro patch clamp recordings of pyramidal neurons from cortical layers II & III has revealed possible Panx1-null phenotypes, including persistent hyperpolarized resting membrane potential, and reduced excitability in response to 4AP. This suggests that Panx1 may play a role in maintaining neuronal excitability, and may be of interest in pathological hyperexcitability. This research provides a valuable tool for understanding the importance of Panx1 in physiological conditions, and quantifies its effects on neuronal excitability.